PREDICTION OF OFF-TARGET SPECIFICITY AND CELL-SPECIFIC FITNESS OF CRISPR-CAS SYSTEM USING ATTENTION BOOSTED DEEP LEARNING AND NETWORK-BASED GENE FEATURE.

CRISPR-Cas is a powerful genome editing technology and has a great potential for in vivo gene therapy.Successful translational application of CRISPR-Cas to biomedicine still faces many safety concerns, including off-target side effect, cell fitness problem after CRISPR-Cas treatment, and on-target genome editing side effect in undesired tissues.To

While the master regulator gene Sry on the mammalian Y chromosome controls the switch for initiating male sex differentiation, many other species rely on environmental factors for gonadal sex differentiation.Yet other species, like zebrafish, appears to rely on a multitude of genetic cues for gonadal sex differentiation.Zebrafish gonadal differenti

Equine coital exanthema (ECE) is a highly contagious, venereally-transmitted mucocutaneous disease, characterized by the formation of papules, vesicles, pustules and ulcers on the external genital organs of mares and stallions, and caused by MAGNEISUM equid alphaherpesvirus 3 (EHV-3).The infection is endemic worldwide and the virus is transmitted m

Abstract Background Chronic rotator cuff pathology (CRCP) is a common shoulder condition causing pain and disability.Physiotherapy is often the first line of management for CRCP yet there is little conclusive evidence to support or refute its effectiveness and no formal evaluation of its cost-effectiveness.Methods/Design This randomised, double-bli

An important goal of smart grid is to leverage modern digital communication infrastructure to help control power systems more effectively.As more and more Internet of Things (IoT) devices with measurement and/or control capability are designed and deployed for a more stable and efficient power system, the role of communication network has become mo